Which of These is HIV?

RTB: HIV tests are poly-reactive and non-standardized; they give no single reaction, and react with no single disease. The list below, from the National Institutes of Health Reference and Reagent Program, is “The Source of Critical HIV Research Materials.” These are what scientists and researchers refer to when they refer to HIV. There is no single particle, no reference particle for HIV tests, and no complete particle.

The proteins and genetic strands are assembled from sub-fragments and partial genetic strands, copied using PCR tests and stitched together with molecular cloning and engineering technologies. Is HIV itself a multiplicity of particles, proteins and events, mistakenly stitched together under one name? Is this why so many illnesses give “positive” reactions on “HIV tests?”

How many different entities are we now collecting under the name “HIV?” Is HIV a catch-all for many activities in the body, some indicating disease, some not? This would explain the diversity and difficulty with HIV testing.

Molecular Clones

HIV-1 Subtype B Panel of SGA Env Clones (Catalog Number 11663)
Env clones of transmitted and early founder HIV-1 subtype B in primary HIV-1 infection (RNA positive, Western blot negative). HIV-1 clade B molecular rev/env clones derived from patient plasma during acute HIV-1 infection. The rev/env genes were PCR amplified by single genome amplification (SGA) followed by directional cloning into pcDNA3.1D. View Catalog 11663 here.

HIV-1 Subtype C Panel of Indian Env Clones (Catalog Number 11672)
Each panel clone represents env/rev sequences from a subject with acute subtype C infection (heterosexual transmission in Pune, India). Each clone expresses a functional env/rev cassette and can be used to generate pseudotyped infectious virions that use CCR5 as the viral coreceptor. The env-pseudotyped virus is part of a standard panel of reference strains for use in neutralizing antibody assays. View the table of available clones here.

HIV-1 Panel of Paired Infant and Maternal Env Molecular Clones (Catalog Number 11674)
A collection of HIV-1 envelope clones isolated from chronically infected women and their infants who were infected with HIV-1 sometime between birth and 6 weeks post delivery. These clones are useful for the study of paired maternal and infant viruses near time of transmission. The infant clones, which represent recently transmitted variants, may also be useful for screening vaccine sera for neutralizing antibodies that can block recently transmitted viruses. View the table of available clones here.

HIV-1 clone DJ263.8 (Catalog Number 11704)
A PCR fragment containing full-length env and rev genes was derived from the genomic DNA of infected PBMC. The original virus obtained from Drs. Francine McCutchan and Vicky Polonis of the US military HIV research program. The env/rev cassette was cloned into CMV/R-MCS expression vector by a directional cloning approach. A single transformed kanamycin resistant E.coli colong was selected and expanded. Recombinant plasmid carries resistance genes for kanamycin. View 11704 here.

HIV-1 clone TV1.29 (Catalog Number 11705)
A PCR fragment containing full-length env and rev genes was derived from the genomic DNA of infected PBMC. The original virus (HIV-1 Tv1) was obtained from Dr. David Montefiori. The env/rev cassette was cloned into pcDNA3.1D/V5-His TOPO expression vector by a directional cloning approach. A single transformed ampicillin resistant E.coli colong was selected and expanded. Recombinant plasmid carries resistance genes for ampicillin and neomycin. View 11705 here.

HIV-1 clone RW020.02 (Catalog Number 11706)
A PCR fragment containing full-length env and rev genes was derived from the genomic DNA of infected PBMC. The original virus obtained from the NIH AIDS Research and Reference Reagent Program. The env/rev cassette was cloned into CMV/R-MCS expression vector by a directional cloning approach. A single transformed kanamycin resistant E.coli colong was selected and expanded. Recombinant plasmid carries resistance genes for kanamycin. View 11706 here.

HIV-1 clone ZA012.29 (Catalog Number 11707)
A PCR fragment containing full-length env and rev genes was derived from the genomic DNA of infected PBMC. The original virus obtained from the NIH AIDS Research and Reference Reagent Program. The env/rev cassette was cloned into CMV/R-MCS expression vector by a directional cloning approach. A single transformed kanamycin resistant E.coli colong was selected and expanded. Recombinant plasmid carries resistance genes for kanamycin. View 11707 here.

Monoclonal Antibodies

Mouse anti-human a4-ß7 integrin (Catalog Number 11718)
This monoclonal antibody reacts with a unique epitope of a4ß7 integrin such that it does not block the binding of commercially available a4 integrin or ß7 integrin single chain specific monoclonal antibodies. View catalog number 11718 here.

Peptides

HCMV pp65 Peptide Pool(Catalog Number 11549)
138 peptide sequences (15-mers) spanning the entire amino acid sequence of the HCMV pp65 protein, overlapping by 11 amino acid residues. View catalog number 11549 here.

HIV-1 PTE Peptides (Env, Nef, Pol, and Gag) (Catalog Numbers 11551-11554)
This peptide panel is designed to permit expression of the most frequent potential T cell epitopes (PTE) embedded in the sequences of circulating HIV-1 strains of HIV-1 worldwide. The peptides are 15 a.a. in length and contain naturally occurring 9 a.a. sequences that are potential T cell determinants, captured in an unbiased manner (1). Its use is intended for T cell assays (e.g., IFN-? ELISpot, intracellular cytokine staining by flow cytometry) measuring HIV-1-specific responses (2), particularly in vaccine trials. View 11551 here, 11552 here, 11553 here, and 11554 here.

Polyclonal Antibodies

Anti-human Bst-2 (Tetherin) (Catalog Number 11721)
Antibody was made against purified recombinant Bst-2 from E.coli. The antibody recognizes endogenous protein and exogenously expressed protein. Glycosylated and non-glycosylated forms of Bst-2 are recognized in western. View Catalog 11721 here.

Proteins

Fc-gp120 JRCSF (Catalog Number 11556)
Fc-gp120 chimera was constructed by fusing human IgG1 Fc domain N-terminal to gp120 from HIV-1 JR-CSF strain. Fc was fused in-frame to the Leu residue at position 51. Protein was expressed in CHO cells and purified by protein A affinity chromatography. View Catalog Number 11556 here.

Griffithsin (Catalog Number 11610)
Novel anti-HIV-1 protein, isolated from Griffithsia sp. This protein is active against HIV-1, HIV-2, SIV and the SARS coronavirus. GRFT has been shown to be directly virucidal and prevents viral fusion and entry via direct association with oligosaccharides present on HIV gp120 and gp41 (Mori et al., 2005). View Catalog Number 11610 here.

Viruses

SHIV-1157ipd3N4 (1) , (Catalog #11689)
SHIV-1157ipd3N4 (1) is a molecular clone of a simian-human immunodeficiency virus (SHIV) which encodes the envelope gene of an HIV clade C strain derived from a 6-month-old Zambian infant. The original construct, the infectious molecular clone SHIV-1157i (2), was adapted by serial passage in five monkeys. SHIV-1157ipd3N4 was generated by directly cloning the 3′ half of a virus isolated from a monkey that developed AIDS (absolute CD4+ T cells <200 cell/?l). An extra NF- ?B binding site was engineered into the 3? LTR of the proviral construct; this duplication is copied into the 5? LTR during the subsequent round of viral replication. The resulting SHIV-1157ipd3N4 has increased replicative capacity in the presence of tumor necrosis factor-a (TNF-?); for expanding viral stocks, the addition of TNF-? is recommended at 10 ng/ml (1). View Catalog Number 11689 here.

HIV-1 AD.MDR01, (Catalog #11700)
This virus is an isolate from a newly infected patient associated with multi-drug resistance and rapid disease progression. View Catalog Number 11700 here.

https://www.aidsreagent.org/

11 thoughts on “Which of These is HIV?

  1. These are what scientists and researchers refer to when they refer to HIV,

    Either they’re all HIV, or none of them are HIV.

  2. Or, HIV is many things.

    I have to say, I find this interesting. It’s more than a little wonky, and not easy for prime time, but you can get a sense of how hard these guys have to work to cobble together these molecules. I’m more than fairly convinced that there are several dozen-to-innumerable processes going on at a cellular level, that are now being categorized (and synthesized) as a single idea.

    That’s why I’m more than a little sure that Peter D. is incorrect, and the Perth group is more than a little correct. HIV isn’t a ‘harmless passenger virus’ – it’s not a singularity. It’s a multiplicity of events, synthesized into one idea…

  3. “Peter D. is incorrect” What does that even mean ? Could you be more bogus and over-generalized ? Nobody would even be listening to the Perth Group if it wasn’t for Duesburg.

    Try to be accurate, and have a little respect, d——, Peter has sacrificed more than you ever will to get at the truth.

  4. RTB’s position is that Duesberg’s theory is incorrect in many details, and Perth may be more correct, but nobody knows everything.

    We’re not molecular biologists, but the tests, the drugs and the stigma are a civil rights issue.

  5. Frankly,

    I hope you won’t think I’m picking on you, but I need to point this out. I understand your admiration for Peter Duesberg. But, consider for a moment if he would be as defensive because someone disagreed with him. The whole point of the dissidence movement is criticism of a prevailing theory. For myself, the very heart of my discomfiture with the AIDS establishment is their unwillingness to tolerate disagreement and dissent. It’s bad science. It’s a betrayal of the scientific method to say that any body of scientific research is set in stone and cannot be retested, criticized, revised, and even, disproved. It shouldn’t be personal or political. It should be no crime in science to be wrong. All that matters is the advancement and evolution of knowledge. It’s all part of the process. No theory and no theorist should be put on such a pedestal. That way lies madness.

  6. Is it the medications that one is given when one is supposedly diagnosed with having HIV?…or is it any lifestyle, such as drugs, poor living conditions, unprotected sex with many partners (whether male or female)…why have so many died from this HIV/AIDS?..cus right now I have a friend who is in a nursing home who was diagnosed with HIV related meningitis..SO WHAT IS REALLY GOING ON?..

  7. Please read the page on HIV “Testing”, and please visit QuestioningAIDS.com, or any of the many facebook pages (AIDS can be healed, HIV-AIDS Truth and Reconciliation), etc, and pose your questions.

    Please though, first, read about HIV testing. You will understand more by doing that than almost any other thing.

    http://reducetheburden.org/?page_id=13

  8. The friend who was in the nursing home battling “hiv related” meningitis, has died..but I must say..he fell out his bed and bumped his head and had a siezure and never pulled through..

  9. Were not people dying of this “HIV/AIDS” way before the Medications were produced?…and I read also that HIV may not be real, but AIDS is…Im understanding that AIDS is not a disease within itself, but any or collection of illnesses that may take over ones body…So if a person stops the “activity” that caused an illness, can they not heal themselves?

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