False Positive or Negative PCR Viral Load in Pregnant Woman in Zaire

HIV Positive, HIV Negative. The ‘twain often meet. HIV tests have no standards, give reactions for dozens to hundreds of known and unknown reasons.

Here a woman in Zaire, described as “immundeficient” with tuburculosis, and pregnant, is put through a ‘viral load’ run-around. She is positive and negative.

And that’s HIV testing, folks. Which is why it should be illegal as a diagnostic tool….

Failure to Quantify Viral Load with Two of the Three Commercial Methods in a Pregnant Woman Harboring an HIV Type 1 Subtype G Strain


AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 14, Number 5, 1998 Mary Ann Liebert, Inc.


The level of HIV-1 RNA in plasma has become one of the most important markers in the follow-up of HIV- infected patients. Three techniques are commercially available: both the Amplicor HIV Monitor and the NASBA HIV-1 RNA QT are target amplification methods, whereas the Quantiplex HIV RNA assay is a branched DNA signal amplification technique. Detection in both target amplification techniques is based on a single primer pair and a single probe in the gag region, whereas multiple probes capture the pol region of the viral RNA in the branched DNA assay.

We investigated the discrepant observation of an undetectable viral load in an immunodeficient pregnant HIV-l-infected patient of African origin with no prior antiretroviral treatment. Although clinical progression was present in this patient with tuberculosis and a low CD4 cell count, viral load determinations with both the Amplicor Monitor and NASBA assays revealed no detectable RNA levels. The presence of HIV-1 RNA in the plasma of the patient was demonstrated by an in-house RNA-PCR. Subsequent HIV-1 RNA quantification with the branched DNA method revealed a high viremia (460,000 copies/ml).

[Note – that’s “undetectable viral load” on one test, and “high viremia” on another. What are these tests measuring? TB? Pregnancy? Wishful thinking?]

DNA sequence analysis of the gag gene identified a subtype G HIV-1 strain (HIV-Ibl)- To our knowledge this is the first report of a patient harboring an HIV-1 genotype of the main group with a high viral load as quantified by the branched DNA assay, but undetectable with the two commercial HIV RNA amplification techniques because of genetic divergence. In the case of discrepant low viral loads determined by one amplification technique in patients with advanced clinical stage one should use an alternative quantification technique for confirmation.

The researchers continue down the rabbit-hole, worrying about their genetic probes, and “genetic divergence” – this is the assumption they’re making in order to spare themselves the obvious, painful realization: These tests do not measure any specific item, quality or quantity, and they should not be used on poor African women who are trying to give birth to healthy children.

One thought on “False Positive or Negative PCR Viral Load in Pregnant Woman in Zaire

  1. I am a chiropractor in Michigan. I have a patient who recently tested hiv +, his doctor did a viral load test, and has him on acylovir. I have been following your work for a few years. I asked my patient when he had tested negative last. It was 6 weeks before he tested positive. He tested positive while he had a pneumonia episode and had been hospitalized. He has asthma, and has been treated for this for many years, and is prone to lung infections. It makes sense to me from reading your work, that his positive test had to do with his body fighting whatever infection he had currently, and his viral load test was simply a way for the doctor to confirm that he needed to be on acyclovir. Of course it would be illegal for me to advise him, since I am not a medical doctor. But I will definitely refer him to your website for his own research. I believe he is healthy and they have trapped him into their HIV web. The man is gay and older, in his late 60s. I wonder if they would have tested him if he were straight and married? Odd too that he has had quite an active sex life for all these years, and just now tests positive when he is quite ill from another type of infection, isn’t it.

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